Plasmid_Backbone

Part:BBa_K625005:Design

Designed by: Michael Eichenberger   Group: iGEM11_ETH_Zurich   (2011-09-21)


pSB6A5


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2722
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal NotI site found at 9
    Illegal NotI site found at 2728
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2722
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found at 2722
    Illegal suffix found at 2
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found at 2722
    Plasmid lacks a suffix.
    Illegal XbaI site found at 2737
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal SapI site found at 771


Design Notes

Find out minimal pBR322 origin


Source

psb4A5, but ORI exchanged with ORI of pSEVA 171

References